Generally, the cell disruption of samples in the lab will take between 15 seconds and 2 minutes. As the intensity of sonication is very easy to adjust by amplitude setting a sonication time as well as by choosing the right equipment, it is possible to disrupt cell membranes very gently or very abruptly, depending on the cell structure and on the purpose of cell disruption.
The temperature during the process can be monitored by an integrated temperature sensor and can be easily controlled by cooling (ice bath or flow cells with cooling jackets) or by sonication in pulsed mode.
During pulse-mode sonication, short sonication burst cycles of 1-15 seconds duration allow for heat dissipation and cooling during the longer intermittent periods. All ultrasound processes are completely reproducible and linearly scalable.
1. Ultrasonic vibration source (drive power): The 50-60Hz mains into high-power high-frequency (15kHz-100kHz) power supply to the transducer.
2. Transducer (controller, transducer): The high-frequency electrical energy into mechanical vibration energy.
3 horn: coupling and fixed transducer and tool head, the transducer amplifies the amplitude sent to the tool head.
4. Tool head (into the rod): the mechanical energy and pressure transmitted to the work, but also amplify the function.
Amplitude/power adjustment range
Volume of reactor
Ultrasonic device + Digital Generator
Stainless steel reactor, Remote communication interface, flow meter
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